Application of Hydrogen-Based Denitrification: Simultaneous Removal of Nitrate and Reactive Black 5 Dye from Textile Wastewater Containing Organic Matter

In this study, a hydrogen-based denitrification (HD) reactor was used to investigate the simultaneous treatment of nitrogen and decolorization in textile wastewater contaminated with organic matter. The operated two phases: without Despite short hydraulic retention time, HD system successfully removed all pollutants, including nitrate, nitrite, reactive black-5 dye chemical oxygen demand. unhindered efficiency for presence pollutants observed. With addition matter, removal increased slightly from 85% 90–100%, rate doubled 25% 50–60%. Organic matter played crucial role stimulating heterotrophic bacteria during biological acted as carbon source facilitating azo bond cleavage degradation. generation toxic byproducts changes dominant microbial community, remained stable improved. This approach offers promising solution enhancing wastewater, providing cost-effective environmentally friendly option developing countries treat before discharge.